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1 Problem (Due Wed., Feb. 17) N-tris(hydroxymethyl)aminomethane (acronym TRIS, structure shown below) is commonly + used as a biochemical buffer. The pKa of the protonated form of TRIS (TRISH ) is 8.08. A laboratory manual has the following recipe for the preparation of 1.00L of .0500M pH 7.80 TRIS Dissolve buffer: 2.08g of TRIS (MW 121.1) and 5.17g of TRIS hydrochloride (MW 157.6) in 500mL of deionized water and dilute to 1.00L. a) Verify that this recipe is correct. b) Calculate the pH of the solution obtained by adding 3.0mL of 1.0M NaOH to 500.0mL of the above buffer. TRIS: (HOCH2)3CNH2

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