Draw out population replacement described in the case study regarding Streptococcus pneumoniaeand levofloxacin resistance. Include in your diagram the following:
A. Draw the systemic infection in circulatory system. Initial population of Streptococcus pneumoniae acquired from a blood draw. Indicate the antimicrobial susceptibility of this predominate population related to the parC subunit of topoisomerase IV and gyrA subunit of topoisomerase II. Also show the mutant strain (at low concentration) that was responsible for the patient’s demise.
B. Draw the systemic infection. Initial effect of levofloxacin treatment on the bacterial population from picture 1. Be sure to provide susceptibility details – how does the single mutant (parC) fair compared to the double mutant (parC; gyrA) to treatment with Levofloxacin?
C. Draw the systemic infection. Streptococcus pneumoniae population acquired from the second blood draw. Indicate the antimicrobial susceptibility of the new predominant
bacterial population and indicate the relevant mutations in the strain.